Performance of the chinese hamster ovary cell hypoxanthine guanine phosphoribosyl transferase gene mutation assay 中国仓鼠卵巢细胞次黄质鸟嘌呤转磷酸核糖基酶基因变异鉴定的操作
2.
2 - e4 and s2 is induced respectively by 8 - ag and 5 - brdu with different drug concentration to make them deficient in hypoxanthine - guanine phosphoribosyl transferase ( hgprt ) and in thymidine kinase ( tk ) respectively and renamed 2 - e4 - a and 82 - 6 . their antibodies " isotypes are tested by goad anti - mouse isotype regent 取驯化好并处于对数生长期的2 - e _ 4 - a和s _ 2 - b细胞,再常规融合和筛选,三次克隆化后得稳定分泌双特异性抗体的杂交-杂交瘤细胞株6株。